Amendment status not verified — confirm the current text below against the official source.
If any difficulty arises in giving effect to the provisions of this Act, the State Government may, by order, published in the Official Gazette, make such provision not inconsistent with the provisions of this Act, as appears to 161 Protection of action taken in good faith. Forfeiture of seed. Power to make rules. Power to remove difficulties. PUNJAB GOVT. GAZ. (EXTRA), NOVEMBER 6, 2020 (KRTK 15, 1942 SAKA) the Government to be necessary or expedient for the purpose of removing the difficulty: Provided that no such order shall be made under this section, after the expiry of a period of three years from the date of commencement of this Act. SCHEDULE (see section 4) DRAFT OF MINIMUM SEED CERTIFICATION STANDARDS FOR TISSUE CULTURE BASED SEED POTATO I. Application and Amplification of General Seed Standards for tissue culture based potato seed (a) The general seed standards are applicable to tissue culture raised mini-tubers (G-0) multiplied through aeroponics or nethouse conditions and further multiplied in the field for four generations (G1-G4) as shown below: Stages of 'Tissue Culture Based Potato Production System' Mother Plant Multiplication through Tissue Culture (8-12) Cuttings/Subculture) G0 Aeroponics G0 Net House G1 Open Field G1 Open Field G2 Open Field G2 Open Field G3 Open Field G3 Open Field G4 Open Field G4 Open Field I. Eligibility requirements for tissue culture based mini-tubers (G-0) The mini-tubers to be eligible for certification shall be from a source meeting the following standards aeroponics or nethouse facilities. (i) Laboratory and aeroponics or nethouse facilities used for production of plantlets/micro-tubers or mini-tubers shall be maintained free of potato pests or vectors of potato pathogens. Failure to keep such pests under control may cause rejection of all lots maintained in the facility. All potting or growth media shall be sterile. Water to be 162 PUNJAB GOVT. GAZ. (EXTRA), NOVEMBER 6, 2020 (KRTK 15, 1942 SAKA) used in a laboratory or aeroponics or nethouse operation should be free from impurities. (ii) Hygienic conditions like maintenance of air purity, sub culturing under laminar flow only, use of pure tissue grade chemicals for media preparation, proper autoclaving of cultured media, proper disposal of contamination etc. shall be strictly observed during micro propagation, potting, planting, irrigating, movement and use of equipment and other laboratory practices to guard against the spread of diseases or pests in the facilities used for seed multiplication. (iii) All micro propagation and aeroponics or nethouse facilities must be approved by authorised State or Central Govt agency, as per the standard/guidelines. These facilities must have a changing area between the double doors. (iv) The aeroponics facility used for mini-tuber production from micro- plants shall be approved by Central Potato research Institute, Shimla (v) The net house must be "insect proof" and be equipped with a double- door entrance, provision for footwear disinfection prior to entering the net house and insect proof ventilation screening on intakes and exhaust openings. The persons entering the aeroponics or nethouse facility should use Wellington boots (Plastic boots) and change lab- coat in the changing area to reduce the chances of inadvertent introduction of vector insects clinging to clothes. (vi) The material being initiated for producing mini-tubers must be of Notified variety and confirmed identity. It must be duly documented with respect to origin. (vii) The plants of a potato varieties being initiated for tissue culture should be tested in an accredited laboratory for freedom from the following PVA (Potato Virus A), PVS (Potato Virus S), PVM (Potato Virus M), PVY (Potato Virus Y), PVX (Potato Virus X), PLRV (Potato Leaf Role Virus), PALCV (Potato Apical Leaf Curl Virus), GBNV (Groundnut Bud Necrosis Virus), PSTVd (Potato Spindle Tuber Viroid) entophytic and Phytoplasma or epiphytic bacteria and fungi. Tests must be carried on a minimum of ten plantlets of each variety selected at random. For virus testing ELISA or an equivalent 163 PUNJAB GOVT. GAZ. (EXTRA), NOVEMBER 6, 2020 (KRTK 15, 1942 SAKA) method should be used, for viroid RT-PCR should be used, and for fungi and bacteria light microscopy and culturing on media should be used.