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Quality Control Test recommended at Broth Stage 3.1. Qualitative Test 3.1.1. Check for free from contaminants by preparing slide and observing under microscope. 3.1.2. The pH of bacterial broth shall normally be between 7.0 to 8.0. 3.1.3. Gram staining test shall be carried out as described in 4.1. 3., and 4.1.3.1 & 4.1.3.2. of Rhizobium of this standard. 3.1.4. See the colour change in the media after 24 hours from inoculation. The colour will change from green to blue. 3.1.5. Watch the pellicle just below the surface of the media. It checked on the third day after keeping inoculated broth undisturbed. 3.2. Quantitative Test 3.2.1. Most Probable Number (MPN) as given in Annexure-E. The counts of Azosprillium in the final broth from shake culture or fermentors shall be not less than 108 to 109 cells / ml. Otherwise the broth should be rejected.