Amendment status not verified — confirm the current text below against the official source.
NITROGEN FIXING BIO-FERTILISERS: - a. RHIZOBIUM INOCULANTS (RI) (IS 8268 - 2001) (i) RI shall be carried based, the colour depending on the colour of the carrier (ii) RI shall contain a minimum of 107 viable Rhizobium cells/g of the carrier on dry - mass basis till 6 months expiry period from the date of manufacture. The number shall be counted by the plate count method as given in 4.2 and 4.3 of 1A of Part B of Schedule-II. (iii) RI shall have a maximum of six months expiry period from the date of manufacture. (iv) RI shall have no contamination with other micro- organisms at 105 dilution when counted as given in 4.3 of 1 A of Part B of Schedule- II. (v) The pH of RI shall be between 6.5 and 7.5 when tested as given in Annexure-A. (vi) RI shall show effectiveness nodulation on all those species and / or cultivars on the packet before the expiry date when tested. If good effectiveness pink nodulation is obtainable in the inoculated species together with total absence or sometimes presence of stray nodules in the controls, it should be concluded that RI contains effective Rhizobium. The total dry mass of inoculated plants shall be significantly higher than that of the un-inoculated controls and at least 50 percent more than the controls. (vii) The carrier material such as peat, lignite, peatsoil, humus favouring growth to be neutralized with calcium carbonate and sterilized shall be in the form of a powder capable of passing through 150 to 212 micron (72 to 100 mesh) IS sieve. (viii) Specified mother culture be obtained from any recognized institution maintaining the mother cultures. The manufacturer may control the quality of the broth, it should get verified at least by two institutions as mentioned below: Note: At present National Bio-fertiliser Development centre and (NBDC), Ghaziabad and its Six Regional Centres located at Bangalore, Bhubaneshwar, Imphal, Hissar, Jabalpur and Nagpur, Indian Agricultural Research Institute (IARI), New Delhi, Tamil Nadu Agricultural University (TNAU), Coimbatore, University of Agricultural Science, Bangalore are sources for supplying the mother culture. (ix) The RI carrier shall be in the form of fribal (moist) with 30-40 percent (m/ m) moisture content when tested as given in Annex-B. (B) AZOTOBACTER CHROOCOCCUM INOCULANTS (AI) (IS 9138 -2002) (i) AI shall be carrier- based, the colour depending on the colour of the carrier. (ii) AI shall contain a minimum of 107 viable Azotobacter cell/g of the carrier on dry - mass basis till 6 months expiry period from the date of manufacture. The number shall be counted by the plate count method as given in 4.2 of 1 A of Para B of Schedule II and Azotobactor Chroococcum colonies are gummy, raised with or without striations, viscous and often sticky. The pigmentation varies from very light brown to black. Count the colony number and observe the cyst formation as given Part B - Schedule II (4.2) and calculate number per gram of the carrier material. (iii) AI shall have a maximum of six months expiry period from the date of manufacture. (iv) AI shall have no contamination with other microorganisms at 105 dilution. (v) The pH of AI shall be between 6.5 and 7.5 when tested as given in -A. (vi) The carrier material such as peat, lignite, peatsoil, humus favouring growth to be neutralized with calcium carbonate and sterilized shall be in the form of a powder capable of passing through 150 to 212 micron (72 to 100 mesh) IS sieve. (vii) Specified mother culture be obtained from any recognized institution maintaining the mother cultures. The manufacturer may control the quality of the broth, it should get verified atleast by two institutions as mentioned in VIII of 1 of Part A of Schedule -I. (viii) The AI carrier shall be in the form of fribal (moist) with 30-40 percent (m/ m) moisture content when tested as given in Annexure-B C. AZOSPIRILLUM INOCULANTS (ASI) (IS 14806-2000) (i) ASI shall contain 107 viable Azospirillum cells / g of the carrier material on dry mass basis. (ii) ASI shall have no contamination with other microorganisms at 105 dilution. ASI contamination in semi solid medium should be checked by semi dilution and spread plate method with solid complete medium. (iii) The pH of ASI shall be 6.5 to 7.5 when tested as given in Annexure-A (iv) The ASI carrier shall be in the form of fribal (moist) with 30-40 percent moisture. When tested as given in Annexure-B (v) ASI shall show effective root development on all cultivars/crops against which the Inoculant is intended to be used. (vi) Specified mother culture be obtained from any institution maintaining the mother cultures. The manufacturer may control the quality of the both, it should get verified atleast by two institutions as mentioned in VIII of I of part A of schedule-I.